Profiling DNA supercoiling domains in vivo
نویسندگان
چکیده
منابع مشابه
Profiling DNA supercoiling domains in vivo
Transitions in DNA structure have the capacity to regulate genes, but have been poorly characterised in eukaryotes due to a lack of appropriate techniques. One important example is DNA supercoiling, which can directly regulate transcription initiation, elongation and coordinated expression of neighbouring genes. DNA supercoiling is the over- or under-winding of the DNA double helix, which occur...
متن کاملDNA supercoiling inhibits DNA knotting
Despite the fact that in living cells DNA molecules are long and highly crowded, they are rarely knotted. DNA knotting interferes with the normal functioning of the DNA and, therefore, molecular mechanisms evolved that maintain the knotting and catenation level below that which would be achieved if the DNA segments could pass randomly through each other. Biochemical experiments with torsionally...
متن کاملDNA knots and DNA supercoiling.
Type II DNA topoisomerases permit passages of double stranded DNA segments through each other and this is achieved via a complex mechanism involving a transient cleavage of one duplex, a passage of the second duplex through the topoisomerase-spanned cleavage site and finally resealing of the cut duplex. Type II DNA topoisomerases facilitate many DNA transactions requiring manipulation of long D...
متن کاملSupercoiling in DNA and chromatin☆
Supercoiling is a fundamental property of DNA and chromatin. It is modulated by polymerase and topoisomerase activities and, through regulated constraint, by DNA/chromatin binding proteins. As a non-covalent and elusive topological modification, supercoiling has proved intractable to research despite being a crucial regulator of nuclear structure and function. Recent studies have improved our u...
متن کاملSupercoiling DNA Locates Mismatches.
We present a method of detecting sequence defects by supercoiling DNA with magnetic tweezers. The method is sensitive to a single mismatched base pair in a DNA sequence of several thousand base pairs. We systematically compare DNA molecules with 0 to 16 adjacent mismatches at 1 M monovalent salt and 3.6 pN force and show that under these conditions, a single plectoneme forms and is stably pinne...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genomics Data
سال: 2014
ISSN: 2213-5960
DOI: 10.1016/j.gdata.2014.07.007